绵羊季节性繁殖相关基因TSHR外显子多态性研究

doi:10.11843/j.issn.0366-6964.2016.07.006

Abstract

Abstract:

The study aimed to investigate the genetic variation of thyroid stimulating hormone receptor (TSHR) gene in Ujumqin sheep and Hu sheep，which had different reproductive performance and oestrous cycle，and the distribution of different haplotypes caused by the polymorphic site and its effects on mRNA and secondary structure of protein.This study provided a theoretical basis for seeking the influence of the mutation on TSHR gene expression and phenotype.Direct sequencing was taken to study genetic variation of the TSHR gene in 172 sheep of 2 Chinese Mongolian sheep strains.6 mutant sites were found and genotyped.Chi-square test for independence was performed to find the genotypes of the 4 SNPs (SNP1，SNP3，SNP4 and SNP5) which were significantly different between the 2 sheep populations（P＜0.01）.The SNP5 was missense mutation，the other sites were synonymous mutations.Linkage disequilibrium analysis was used to screen the 4 sites，complete linkage happened between SNP3 and SNP4，and the other sites present different degrees of interlocking relationship.Bioinformatics analysis revealed that not only the mutations occured in the TSHR，but also different haplotypes could result in the changes of mRNA and the secondary structure of protein.These results indicate that：(1) The genotypes distribution of 4 sites in TSHR gene have significant differences between the 2 sheep populations.They form 6 haplotypes，among which H1(CGCC) is the advantage haplotype in Ujumqin sheep，and H4(TTTG) is the advantage haplotype in Hu sheep.(2) The SNP5 site affects the stability of mRNA and protein secondary structure of TSHR gene，which may be one of the important functional sites with potential phenotypic effects.

CLC Number:

S826
S813.3

XUAN Jun-li，MA Xiao-meng，WANG Hui-hua，CAO Jia-xue，WEI Cai-hong，ZHAO Fu-ping，MA You-ji，ZHANG Li，DU Li-xin. Study on Exon Polymorphism of Seasonal Breeding Related Gene TSHR in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1342-1353.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.07.006

https://www.xmsyxb.com/EN/Y2016/V47/I7/1342

References

［1］MONTGOMERY G W，TATE M L，HENRY H M,et al.The follicle-stimulating hormone receptor and luteinizing hormone receptor genes are closely linked in sheep and deer［J］.J Mol Endocrinol,1995,15(3):259-265.
［2］EBLING F J.Photoperiodic regulation of puberty in seasonal species［J］.Mol Cell Endocrinol，2010，324(1-2)：95-101.
［3］BOCKMANN J，WINTER C，WITTKOWSKI W，et al.Cloning and expression of a brain-derived TSH receptor［J］.Biochem Biophys Res Commun， 1997，238(1)：173-178.
［4］赵赓，高磊，沈敏，等.中国美利奴成母羊促甲状腺激素受体基因的克隆与组织表达［J］.新疆农业科学，2014，51(9)：1718-1727.
ZHAO G，GAO L，SHEN M，et al.Cloning and expression of thyroid-stimulating hormone receptor gene in sheep［J］.Xinjiang Agricultural Sciences，2014，51(9)：1718-1727.(in Chinese)
［5］HANON E A，LINCOLN G A，FUSTIN J M，et al.Ancestral TSH mechanism signals summer in a photoperiodic mammal［J］.Curr Biol，2008，18(15)：1147-1152.
［6］CRISANTI P，OMRI B，HUGHES E，et al.The expression of thyrotropin receptor in the brain［J］.Endocrinology，2001，142(2)：812-822.
［7］RODRÍGUEZ E M，BLÁZQUEZ J L，PASTOR F E，et al.Hypothalamic tanycytes：a key component of brain-endocrine interaction［J］. Int Rev Cytol， 2005，247：89-164.
［8］VIGUIÉ C，BATTAGLIA D F，KRASA H B，et al.Thyroid hormones act primarily within the brain to promote the seasonal inhibition of luteinizing hormone secretion in the ewe［J］.Endocrinology，1999，140(3)：1111-1117.
［9］HANON E A，ROUTLEDGE K，DARDENTE H，et al.Effect of photoperiod on the thyroid-stimulating hormone neuroendocrine system in the European hamster (Cricetus cricetus)［J］J Neuroendocrinol，2010，22(1)：51-55.
［10］冉雪琴，赵星艳，王嘉福，等.贵州矮马（Equus caballus）生长激素受体基因5个单核苷酸位点的多态性研究［J］.畜牧兽医学报，2015，46(11)：1974-1986.
RAN X Q，ZHAO X Y，WANG J F，et al.Polymorphism of five SNPs in exon 10 of growth hormone receptor gene in Guizhou pony(Equus caballus)［J］.Acta Veterinaria et Zootechnica Sinica，2015，46(11)：1974-1986.(in Chinese)
［11］丁月云，朱卫华，薛玮纬，等.安徽地方猪TLR4基因第3外显子的SNP分析［J］. 畜牧兽医学报，2014，45(11)：1767-1774.
DING Y Y，ZHU W H，XUE W W，et al.Single nucleotide polymorphism (SNP) analysis of TLR4 gene exon 3 in Anhui native pig breeds［J］. Acta Veterinaria et Zootechnica Sinica，2014，45(11)：1767-1774.(in Chinese)
［12］国家畜禽遗传资源委员会.中国畜禽遗传资源志•羊志［M］.北京：中国农业出版社，2011.
China National Commission of Animal Genetic Resources.Animal Genetic Resources in China•sheep and goat［M］.Beijing：Chinese Agriculture Press，2011.(in Chinese)
［13］WEBSTER J R，MOENTER S M，BARRELL G K，et al.Role of the thyroid gland in seasonal reproduction.III.Thyroidectomy blocks seasonal suppression of gonadotropin-releasing hormone secretion in sheep［J］.Endocrinology，1991，129(3)：1635-1643.
［14］SCHOENMAKERS N，CHATTERJEE V K.Thyroid gland：TSHR mutations and subclinical congenital hypothyroidism［J］.Nat Rev Endocrinol，2015，11(5)：258-259.
［15］TONG G X，MODY K，WANG Z，et al.Hypothyroidism caused by the combination of two heterozygous mutations：one in the TSH receptor gene the other in the DUOX2 gene［J］.Endocr Pathol.2015，26(4)：315-319.
［16］SATOH M，ASO K，OGIKUBO S，et al.Hypothyroidism caused by the combination of two heterozygous mutations：one in the TSH receptor gene the other in the DUOX2 gene［J］.J Pediatr Endocrinol Metab，2015，28(5-6)：657-661.
［17］KARLSSON A C，SVEMER F，ERIKSSON J，et al.The effect of a mutation in the thyroid stimulating hormone receptor (TSHR) on development，behaviour and TH levels in domesticated chickens［J］.PLoS One，2015，10(6)：e0129040.
［18］曲娟娟，于敏，董蕾，等.SNP在动植物遗传育种中的应用［J］.东北农业大学学报，2011，42(12)：9-13.
QU J J，YU M，DONG L，et al.Applications of SNP in animal and plant genetic breeding［J］.Journal of Northeast Agricultural University，2011，42(12)：9-13.(in Chinese)
［19］高磊，沈敏，赵赓，等.绵羊TSHR 基因第10外显子T481C位点多态性分析［J］.农业生物技术学报，2016，24(1)：61-67.
GAO L，SHEN M，ZHAO G，et al.Polymorphism analysis of T481C locus of TSHR gene exon 10 in sheep (Ovis aries)［J］. Journal of Agricultural Biotechnology，2016，24(1)：61-67.(in Chinese)
［20］GURYEV V，SMITS B M，VAN DE BELT J，et al.Haplotype block structure is conserved across mammals［J］.PLoS Genet，2006，2(7)：e121.
［21］ALBERTI L，PROVERBIO M C，COSTAGLIOLA S，et al.Germline mutations of TSH receptor gene as cause of nonautoimmune subclinical hypothyroidism［J］. J Clin Endocrinol Metab， 2002，87(6)：2549-2555.
［22］LIU Y，CHU A，CHAKROUN I，et al.Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation［J］.Nucleic Acids Res，2010，38(20)：6857-6871.
［23］黄冬维.山羊繁殖季节性调控通路相关基因的克隆、多态与表达研究［D］.北京：中国农业科学院，2012.
HUANG D W.Study on cloning，polymorphisms and expression of genes related with seasonal reproduction pathways in goats［D］.Beijing：Chinese Academy of Agricultural Sciences，2012.(in Chinese)

[1]	LUAN Zhao-jin，HE Jian-ning，CHENG Ming，LIU Kai-dong，QU Xu-xian，LIU Nan. Association of the MyoG and PID1 Gene Expression with Intramuscular Fat Content in Sheep Muscles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1986-1994.
[2]	DUAN Xin-chong，WEI Yan-hui，LI Yang，LI Xiang-yun，ZHOU Rong-yan，XI Jian-zhong. Analyzing the Differential Expression of microRNAs in Estrus and Diestrus of Small Tailed Han Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1324-1332.
[3]	MA Xiao-meng，XUAN Jun-li，WANG Hui-hua，YUAN Ze-hu，WU Ming-ming，ZHU Cai-ye，LIU Rui-zao，WEI Cai-hong，ZHAO Fu-ping，ZHANG Li，DU Li-xin. TXNRD1 Gene Polymorphisms and Its Association with Growth Traits in Ujumqin Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 909-921.
[4]	YUAN Ze-hu，WANG Hui-hua，HU Shi-jin，ZHU Cai-ye，ZHAO Fu-ping，ZHANG Li，DU Li-xin，WEI Cai-hong. Population Structure Analysis of Chinese Indigenous Sheep by 50K Chip Data [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 899-908.
[5]	CHEN Xiao-yong，SUN Hong-xin，YUAN Ming，KANG Zhen-jiang，DUN Wei-tao，ZHAO Xing-bo. Association Analyses between Mitochondrial Coding Gene Variations and Sheep Litter Size [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 449-456.
[6]	LIU Rui-zao，LIU Zhen，WU Ming-ming，WANG Hui-hua，ZHU Cai-ye，ZHANG Li，ZHAO Fu-ping，WEI Cai-hong，DU Li-xin. Expression Trend of BTG2/3 in the Mid to Late Embryonic Skeletal Muscle of Sheep and the Relationship between BTG2/3 and MSTN [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1916-1923.
[7]	SA Ru-li，MU Qi-er，WANG Cui-fang，BAO Ling-ling，AO Chang-jin，WANG Si-zhen. Effect of Allium Mongolicum Regel Flavonoids on Lymphocyte Proliferation，mRNA Expression of IL-2，IL-4， IFN-γ in Peripheral Blood Lymphocyte of Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1063-1070.
[8]	WANG Pei，FAN Yan-ru，JIN Xin，YANG Yin-feng. Effects of Bacillus subtilis on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 760-767.
[9]	ZHANG Chun-xiang，GUO Yun-yan，BAI Yuan-sheng,YANG Zi-sen，ZHANG Guo-lin，JIAO Guang-yue，REN You-she. Clone，Bioinformatics Analysis and Expression Characteristics of Ovine GPx5 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1075-1083.
[10]	LIU Qiang，HE Zhi-rui，WANG Yin-long，WU RE lihazi，SAI Wujiafu. Construction Eukaryotic Expression Vector and Its Expression of PLCζ Gene from Chinese Merino Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 541-546.
[11]	ZHOU Ming-liang, ZHANG Xiang-yu, QI Song-zhi, WANG Qi, WU Deng-jun,YANG Ping-gui. Expression and Association between SNPs and Growth Traits of IGF-Ⅰ Gene in Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1363-1370.
[12]	WEI Lin-lin, GAO Jin-sheng, WANG Jing-lin, CHENG Li-fen, QIAO Li-ying, JIA Xia-li, ZHANG Jing, YANG Kai-jie, LIU Jian-hua, LIANG Chen, LIU Wen-zhong. Study on the Cloning and Ontogenetic mRNA Expression of Ovine LPIN1 Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1371-1379.
[13]	RONG En-guang, QIAO Shu-pei, YU Lei, YAN Xiao-hong,YANG Hua, LI Hui, WANG Ning. Cloning and Expression Characterization of Sheep POU2F3 Gene and Its Isoforms in Chinese Merino Skin [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1189-1197.
[14]	LI Da,SUN Wei, SU Rui, ZHANG Zhan-ying, MA Yue-hui, ZHANG You-fa,CHEN Ling, WU Wen-zhong, ZHOU Hong. Molecular Cloning and Construction of Eukaryotic Expression Vector and Mutant Vector of Sheep YAP1 Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1198-1204.
[15]	LIU Bao-feng, ZHOU Sha-sha, WANG Jing-lin, WEI Lin-lin, QIAO Li-ying, LIU Jian-hua, LIANG Chen, LIU Wen-zhong. Differential Expression of Lep and LEPR mRNA in Adipose Tissues of Sheep with Divergent Fat-tails [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1014-1022.

Study on Exon Polymorphism of Seasonal Breeding Related Gene TSHR in Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments